Anti-phospho-DARPP-32 (Thr75)

Anti-DARPP-32 pT75 antibody detects rat DARPP-32. Anti-DARPP-32 pT75 antibody was prepared from monospecific antiserum by immunoaffinity chromatography using phospho peptide coupled to agarose beads followed by solid phase adsorption(s) against non-phospho peptide and non-specific peptide to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum. This antibody is specific for phosphorylated DARPP-32 pT75 . Minimal reactivity occurs against non-phosphorylated DARPP-32 pT75 . Reactivity is completely blocked by treatment with ?-phosphatase. Reactivity against DARPP-32 pT75 occurs from rat sources. However, reactivity is also expected against bovine, canine, human, mouse and NHP sources. Protein function: Inhibitor of protein-phosphatase 1. [The UniProt Consortium]