Anti-EGFR (Phospho-Tyr1173) Rabbit Monoclonal Antibody (Clone RM269)

Epidermal growth factor receptor (EGFR), also known as HER1 and ERBB1, is a cell surface receptor and member of the EGF family of receptor tyrosine kinases with roles in cell proliferation, differentiation, and survival. It is a transmembrane receptor composed of an intracellular tyrosine kinase domain, a transmembrane lipophilic segment, and an extracellular domain that is expressed in epithelial, mesenchymal, and neuronal tissues. Under unstimulated conditions, EGFR is an auto-inhibited monomer in the plasma membrane. Upon canonical ligand binding, EGFR undergoes homodimerization or heterodimerization with HER2, HER3, or HER4, which induces a conformational change in the cytoplasmic domain that facilitates autophosphorylation and intracellular signaling. EGFR contains five C-terminal autophosphorylation sites, tyrosine 1068 (Tyr1068), Tyr1148, Tyr1173, Tyr1086, and Tyr992. EGFR autophosphorylation at Tyr1173 (phospho-Tyr1173) leads to interaction with phospholipase Cgamma (PLCgamma) and Shc, which have roles in activating the MAPK signaling pathway. Increased levels of EGFR (phospho-Tyr1173) are associated with poor progression-free survival in patients with non-small cell lung cancer (NSCLC). Levels of EGFR (phospho-Tyr1173) are increased in, and positively correlate with activation of MAPK signaling and cytokine production in, bronchial epithelial biopsies from healthy individuals exposed to diesel exhaust. Cayman's EGFR (Phospho-Tyr1173) Rabbit Monoclonal Antibody (Clone RM269) can be used for immunohistochemistry (IHC) and Western blot (WB) applications.Synonyms: Epidermal Growth Factor Receptor, ErbB-1, HER1. Immunogen: Peptide corresponding to human EGFR (phospho-Tyr1173). Clone Designation: RM269. Formulation: (Request formulation change), 100 µl of protein A-affinity purified monoclonal antibody. Storage Buffer: PBS, with 50% glycerol, 1% BSA, and 0.09% sodium azide. Host: Rabbit. Isotype: IgG. Applications: IHC, WB. Cross Reactivity: (+) EGFR (phospho-Tyr1173), (-) EGFR without phosphorylation at Tyr1173.