Anti-acetyl-p53 (Lys373 and Lys382) (Tumor Suppressor Protein, Oncogene Protein)

The tumor suppressor p53 is a critical mediator of cellular responses to DNA damage. p53 stability and transcriptional activity are modulated by post-translational modifications such as phosphorylation and acetylation. In response to UV or gamma irradiation, the p53 tetramerization domain becomes a target for acetylation by the transcription factors CREB binding protein (CBP)/p300 and PCAF, which also function as co-activators for p53-mediated transcriptional activation. p300, which possesses intrinsic acetyl transferase activity, specifically acetylates p53 on lysine residue 373 and possibly lysine 382. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:250-1:500, Detects p53-GST acetylated by recombinant PCAF, Active (Cat# P3114) in an in vitro acetyl transferase assay. In vitro acetyl transferase reaction products were resolved by electrophoresis, transferred to nitrocellulose and probed with P1001-30C. Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP* and a chemiluminescence detection system. Note: The molecular weight of GST is 25.5kD. Consequently, the p53-GST fusion protein is detected at ~80kD., Optimal dilutions to be determined by the researcher. *Recommended Secondary Antibodies I1904-39: IgG, X-Adsorbed (HRP) Pab Gt xRb, I1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt xRb, I1904-46Q: IgG, H&L (HRP) Pab Gt xRb, Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.