Anti-Tyrosine Hydroxylase (TYH)

Human TYH (EC 1.14.16.2) is a 58.523 kD protein (528aa) responsible for the enzymatic conversion of L-tyrosine to L-DOPA (dihydroxyphenylalanine). This enzyme is expressed in all catecholaminergic neurons of the CNS and PNS. In the CNS, TYH-positive neurons can be found within the substantia nigra, ventral tegmental area, locus ceruleus, and hypothalamus. In the PNS, TYH-positive neurons can be found within the sympathetic chain, pre-vertebral ganglia and the adrenal medulla. Applications: Suitable for use in Immunohistochemistry, Immunocytochemistry and Western Blot. Other applications have not been tested. , Recommended Dilutions: Immunohistochemistry: 1:1000-1:2000 using 2% paraformaldehyde tissues, Immunocytochemistry: 1:1000-1:2000 using 2% paraformaldehyde cells, Western Blot: 1:2000-1:5000, Optimal dilutions to be determined by the researcher. Recommended Secondary Antibodies: I1906 IgY, Chicken Pab Rb x Ch , I1906-10 IgY, Chicken (HRP) Rb x Ch , I1906-12 IgY, Chicken (FITC) Rb x Ch , I1906-14 IgY, Chicken (AP) Rb x Ch, Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.