Anti-Transforming Growth Factor beta 1 (TGFb1, TGF-beta-1, TGFB1, TGFB)

TGFb1 (Transforming Growth Factor-beta1) is a family of multifunctional 25kD proteins. TGFb1 was originally identified for its ability to induce the growth of normal rodent fibroblasts in soft agar. It is now known that TGFb1 is a potent growth inhibitor of many normal and transformed cell lines. It regulates normal cell growth, development and tissue remodeling following injury. TGFb1 is produced as latent high molecular weight complexes. The activation of latent TGFb1 is an important step in the regulation of its action. Applications: Suitable for use in ELISA, Western Blot, Flow Cytometry and Immunohistochemistry. Other applications have not been tested. Recommended Dilutions: Flow Cytometry: 10ul labels 1x10e6 cells in 100ul. Membrane permeabilisation is required., Immunohistochemistry: Frozen sections, Immunohistochemistry (Paraffin): Has been successfully used on FFPE tissue without pre-treatment. Can use citrate, pH 6, EDTA, pH 8, or pepsin mediated antigen retrival., Optimal dilutions to be determined by the researcher. Recommended Secondary Antibodies: I1904-04D: IgG, H&L (AP) Pab Gt xMo, I1904-06F: IgG, H&L (FITC) Pab Gt xMo, I1904-06C: IgG, H&L (HRP) Pab Gt xMo, Recommended Negative Control: I1904-78R: IgG1 Murine Negative Control, Immunohistochemistry Postivie Control: Human Breat Carcinoma, Hybridoma: SP2/0-Ag 14 myeloma cells with spleen cells from Balb/c mice. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.