Anti-TOR, phosphorylated (S2448) (Mammalian Target of Rapamycin, FRAP1, FRAP2, MTOR, RAFT1)

The mammalian Target of Rapamycin (mTOR) is a member of the PI 3­kinase­related kinase (PIKK) family. mTOR is the protein target of rapamycin, an anti­rejection drug used in transplantation and promising anti­cancer agent. Recent evidence suggests that mTOR is phosphorylated at S2448 by the S6 protein kinase 1 (S6K1) (1, 2), rather than a direct phosphorylation by the Akt protein kinase. This S2448 phosphorylation activates mTOR to affect the downstream control of cell growth and survival. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1ug/ml, Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.