Anti-TBK1 (Clone 4E6)

TANK-binding kinase 1 (TBK1) is a non-canonical inhibitor of NF-kappaB kinase (IKK) that has an essential role in regulating inflammatory responses to pathogens. Following activation of toll-like receptors by viral DNA, TBK1 interacts with various partners such as STING, MAVS, and TANK to phosphorylate and activate interferon regulatory factors (IRFs) 3 and 7 as well as DEAD-box helicase 3 X-linked (DDX3X), which leads to transcriptional activation of pro-inflammatory and antiviral genes including interferon (IFN) subtypes alpha and beta. TBK1 induces nuclear translocation of NF-kappaB to initiate a pro-inflammatory response via phosphorylation of NF-kappaB inhibitor alpha (NFkappaBIalpha), IKKbeta, or NF-kappaB p65 subunit (RelA). Cytosolic localization of E. coli, Salmonella, and S. pyogenes increases in TBK1-/- murine embryonic fibroblasts, macrophages, and epithelial cells, suggesting TBK1 maintains vacuolar integrity, which is critical to bacterial clearance. TBK1 phosphorylates the autophagy receptor optineurin to enhance binding of ubiquitin-like microtubule-associated protein light chain 3 (LC3) modifiers and induce autophagic clearance of S. enterica. It also binds to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) non-structural protein 13 (Nsp13), a helicase-triphosphatase and component of the viral replicase-transcriptase complex. Cayman's TBK1 Monoclonal Antibody (Clone 4E6) can be used for ELISA, Immunohistochemistry (IHC), and Western blot (WB) applications. The antibody recognizes TBK1 at approximately 84 kDa.Synonyms: NF-kappaB-Activating Kinase, Serine/Threonine-Protein Kinase TBK1, TANK-Binding Kinase 1. Immunogen: Full length human recombinant TBK1 protein. Clone Designation: 4E6. Formulation: (Request formulation change), 100 µg of protein G-purified monoclonal antibody. Storage Buffer: PBS, pH 7.2, with 50% glycerol and 0.02% sodium azide. Host: Mouse. Isotype: IgG1. Applications: ELISA, IHC, and WB. UniProt Accession #: Q9UHD2.