Anti-TBB1 (Tubulin beta-1 chain, TUBB1) (Azide Free) (Biotin)

The tubulin family of globular proteins has several members, the most common of which are a-tubulin and ß-tubulin, proteins which make up microtubules of the cytoskeltons of probably all eukaryotic cells Except in the simplest eukaryotes, tubulin (100kD) exists in all cells as a heterodimer of two similar but non-identical polypeptides (55kD each), designated alpha and beta. Within either family of alpha/beta tubulin heterodimers, individual subunits diverge from each other (both within and across species) at less than 10% of the amino acid positions. The most extreme diversity is localized to the carboxyl-terminal 15 residues. Delta (d) and epsilon (e) tubulin have been found to localize at centrioles and may play a role in forming the mitotic spindle during mitosis, though neither is as well-studied as the a- and ß- forms. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: ELISA: 1:1,000, Western Blot: 1:100-1:500, Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. , , Note: Applications are based on unconjugated antibody.