Anti-sPLA2 (human Type IIA) Antiserum

sPLA2 catalyzes the hydrolysis of phospholipids at the sn-2 position yielding a free fatty acid and a lysophospholipid. The release of arachidonic acid from membrane phospholipids by PLA2 is believed to be a key step in the control of eicosanoid production within the cell. Human sPLA2 (Type IIA) is secreted, 14 kDa protein which is dependent on Ca2+ for optimal activity. Human sPLA2 (Type IIA) is 79% homologous to rat platelet and murine Type II sPLA2s. Cayman's sPLA2 antibody detects a band of approximately 15 kD by Western blot. For other applications, the working concentration of the antiserum must be determined empiricallySynonyms: GIIC sPLA2, Group IIA Phospholipase A2, NPS-PLA2, Phosphatidylcholine 2-Acylhydrolase 2A, Phospholipase A2 Membrane Associated. Immunogen: Human recombinant synovial sPLA2 (Type IIA). Formulation: (Request formulation change), 100 µl of Lyophilized antiserum. Storage Buffer: Lyophilized from serum. Reconstitute in 100 µl of double distilled water. Host: Rabbit. Applications: WB. Cross Reactivity: (-) sPLA2 (Type V). UniProt Accession #: P14555. Origin: Animal/Rabbit.