Anti-SLP 76 (LCP2, SLP76, SLP-76, lymphocyte cytosolic protein 2 (SH2 domain containing leukocyte pr

Alternate Name: LCP2, SLP76, SLP-76, lymphocyte cytosolic protein 2 (SH2 domain containing leukocyte protein of 76kD), 76 kD tyrosine phosphoprotein, SH2 domain-containing leukocyte protein of 76kD, lymphocyte cytosolic protein 2 (SH2 domain containing leukocyte protein of 76kD), Domain Information: KOG1984: Vesicle coat complex COPII, subunit SFB3 [Intracellular trafficking, secretion, and vesicular transport], smart00252: Src homology 2 domains, smart00454: Sterile alpha motif , Gene Ontology Terms: immune response, intracellular signaling cascade, protein binding, transmembrane receptor protein tyrosine kinase signaling pathway, Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: ELISA: 1:32,000, Western Blot: 0.5-2ug/ml ~75kD band observed in Jurkat lysates., Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Peptide Blocking:, Corresponding peptide is available for Peptide Blocking studies. See S1014-20K-P. Antibody is typically 0.5mg/ml and peptide is supplied as a 100ul pellet. When peptide is reconstituted in 200ul water, the concentration would also be 0.5mg/ml. To start, the best ratio would be 1:1 (molar excess of peptide relative to antibody when identical volumes are mixed). Mix equal volumes of peptide and antibody at the required dilution and leave at ambient temperature. It is best is to have two identical blots to be incubated with equal amount of antibody, but one with the antibody pre-adsorbed to the peptide for 20min. Then incubate and develop the two blots in parallel.