Anti-SIRT 5 (Sirtuin 5, Sirtuin-5, NAD-dependent Deacetylase Sirtuin-5, SIR2-like Protein 5 SIR2L5)

Sirtuin 5 (SIRT5, SIR2-like protein 5) is a 28-35kD class III member of the sirtuin protein family. It is found in the mitochondrial matrix and is involved in ammonia detoxification. In particular, it deacetylates carbamoyl phosphate synthetase 1/CPS1, thus activating it and allowing for ammonia condensation with bicarbonate and ATP. This is a crucial step in urea formation during prolonged fasting or the use of a high-protein diet. Human SIRT5 is 310aa in length. It possesses a sertuin-type deacetylase domain (aa41-309) that contains an NAD binding motif (Thr276:Glu277:Pro292). There are three potential isoform variants that show aa substitutions at the C-terminus. One contains a 14aa substitution for aa286-310, a second shows a 15aa substitution for aa287-310, and the third shows a 17aa substitution for aa207-310. Full-length human and mouse SIRT5 share 86% aa identity. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilutions: Western Blot: 0.1-0.2ug/ml, Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile TBS, 0.1% BSA. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.