Anti-SALL4 (Sal-Like 4, DJ1112F19.1, DRRS, HSAL4, MGC133050, ZNF797, Cys2His2 Zinc Finger Domain Fam

SALL4 (SAL-like protein 4) is a 1324aa member of the SAL protein family that possess multiple C2H2-type zinc-fingers. It is a transcriptional repressor when associated with histone deacetylase and a transcriptional activator of the Wnt pathway in its native form. It contributes to generation of induced pluripotent stem cells and to axis formation during development. It is frequently overexpressed in germ cell tumors and acute myeloid leukemias. Mutations of human SALL4 can result in conditions such as Okihiro, Duane Radial Ray, or Holt-Oram syndromes. The region used as an immunogen is common to both SALL4 and the ubiquitous 617aa isoform, SALL4B, it shares 76% aa identity between human and mouse. Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 0.025ug/ml, Immunohistochemistry: 25ug/ml, Optimal dilutions to be determined by the researcher. Hybridoma: Myeloma cells with B cells from Balb/c mice. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.