Anti-RIG-I (Clone 1E3)

Retinoic acid-inducible gene I (RIG-I), also known as DDX58, is a cytosolic DExD/H-box RNA helicase and an immune sensing receptor encoded by DDX58 in humans. It is composed of a C-terminal repressor domain, a central RNA helicase domain, and two N-terminal caspase recruitment domains (CARDs). Following recognition of viral dsRNA by the C-terminal and helicase domains, the helicase domain induces an ATP-dependent conformational change, allowing for interaction of the CARD domains with mitochondrial antiviral-signaling protein (MAVS) and induction of a type I interferon (IFN) response. RIG-I levels are elevated in the epidermis of patients with psoriasis. DDX58 expression is decreased in tumor tissues from patients with hepatocellular carcinoma (HCC) compared to nontumor hepatic tissue, and low DDX58 expression is associated with poor disease prognosis. Cayman's RIG-I Monoclonal Antibody (Clone 1E3) can be used for Western blot, ELISA, and immunohistochemistry applications. The antibody recognizes RIG-I at 106.6 kDa from human and mouse samples.Synonyms: Probable ATP-dependent RNA Helicase DDX58, DEAD Box Protein 58, Retinoic Acid-inducible Gene 1 Protein, RIG-I-like Receptor 1. Immunogen: Recombinant human RIG-I protein AA 232-794. Clone Designation: 1E3. MW: 106.6 kDa. Formulation: (Request formulation change), 100 µg of protein G-purified antibody. Storage Buffer: PBS, pH 7.2, with 50% glycerol and 0.02% sodium azide. Host: Mouse. Isotype: IgG2b. Applications: ELISA, IHC, WB. UniProt Accession #: O95786.