Anti-PSMB9 (LMP2, Large Multifunctional Peptidase 2, Proteasome Subunit beta-1i, RING12)

LMP2 (large multifunctional peptidase 2), also called PSMB9 (proteasome subunit beta 9) or PSMB6i, is a 22kD broad­specificity aminopeptidase of the T1B family that is a component of the 20S immunoproteasome core (a7b7b7a7) of the 26S proteasome particle. Induction of LMP2 by IFN­g, IRF­1, TNF­a, CD40L, or heat shock in immune­responsive cells or cancer cells promotes replacement of proteasome subunit PSMB6 by LMP2 and processing of peptides for presentation by MHC I. After auto­cleavage between amino acids (aa) 20­21, human LMP2 (aa21­219) shares 91% aa sequence identity with mouse and rat LMP2. Alternate splicing creates a short form, LMP2.S, which lacks aa4­13 within the propeptide. Applications: Suitable for use in ELISA, Western Blot, and Immunocytochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1ug/ml, Immunocytochemistry: 8­25ug/ml, Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.