Anti-Presenilin Enhancer Protein 2 (PEN2, PEN-2, Gamma-secretase Subunit PEN-2, MDS033, MSTP064, PSE

Presenilin Enhancer 2 (PEN2) is a small integral membrane glycoprotein that contains two recognized transmembrane domains. Both the N- and C-terminal domains are oriented into the lumen of the endoplasmic reticulum (1). PEN2, along with Presenilin 1, Presenilin 2, Nicastrin, and APH-1 form the protein complex y-secretase (2). The proteinase BACE catalyses the initial step in APP processing by cleaving and releasing soluble APPb (3). The remaining membrane bound APP is then cleaved by the y-secretase complex, causing the release of amyloid b-peptide, the main constituent of amyloid plaques. These plaques are a hallmark of Alzheimer's disease pathology (2). In addition to APP, the y-secretase complex cleaves several other proteins and necessary presenilin-dependent signaling cascades, including the Notch pathway (4). It was found that PEN2 is an important part of the y-secretase complex, and knocking it down results in reduced amounts of the complex, resulting in a loss of y-secretase activity (5). Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:1000, Incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight., Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.