Anti-Phosphoinositide 3 Kinase, p55 gamma (PI-3 Kinase p55 gamma, Phosphoinositide-3-kinase Regulato

p55g (Protein of 55kD, gamma subunit, also PI 3-Kinase regulatory subunit gamma and p55 PIK) is a 55-57kD member of the PI 3K p85 family of regulatory subunits. It is widely expressed and serves as a recruitment subunit for the p110 catalytic subunit of PI 3-Kinase. p55g/p55 PIK should not be confused with the 55kD alpha splice variant of the p85 alpha gene (75% aa identity). Human p55g is 461aa in length and contains a unique 34aa N-terminus that binds Rb and prevents cycle progression, followed by a Pro-rich region (aa35-44) and two SH2 domains that bind the p110 catalytic subunit (aa65-160 and 358-452). Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1ug/ml, Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS, sterile 40-50% glycerol, 0.02% sodium azide, aliquot and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.