Anti-PDCD4, CT (Programmed Cell Death 4, Neoplastic Transformation Inhibitor)

Apoptosis, also known as programmed cell death, plays major roles in development and normal tissue turnover in addition to tumor formation (1 for review). During this process, the expression patterns of numerous genes are radically altered. One such gene is the programmed cell death protein 4 (PDCD4), whose expression was found to be upregulated in all cell lines following the onset of apoptosis (2). PDCD4 encodes a tumor suppressor protein whose expression is lost in carcinomas of breast, colon, lung and prostate (3). It can bind to and inhibit the helicase activity of the eukaryotic translation initiation factor 4A (4) and inhibit the transactivation and transformation mediated by the transcription factor AP-1 (5). The kinase Akt regulates PDCD4 by phosphorylation, decreasing the ability of PDCD4 to interfere with the transactivation of AP-1-responsive promoter by c-Jun (6). There are two known isoforms of PDCD4. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 0.5-1ug/ml. See P3120-68A for control peptide., Optimal dilutions to be determined by the researcher. Positive Control: A-20 cell lysate, Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.