Anti-PCK1 (Phosphoenolpyruvate Carboxykinase 1, PEPCK1, PEPCK-C)

PCK1 (Phosphoenolpyruvate carboxykinase 1, also PEPCK­C [cytosolic]) is a monomeric, 67­68kD member of the PEP carboxykinase family of enzymes. It is expressed in postnatal cells such as mammary epithelium, white and brown adipocytes, skeletal muscle cells and hepatocytes. PCK1 has multiple functions, some of which are cell­specific. In particular, PCK1 has both cataplerotic (Greek: to fill down, or remove) and anaplerotic (to fill up, or replace) activity, where it removes and replaces elements of the TCA cycle. It is also gluconeogenic, and promotes glucose formation via PEP generation. Finally, it is glyceroneogenic, creating glycerol­3­ phosphate that is used to reesterify and store just­released free fatty acids in adipocytes. Mouse PCK1 is 622 amino acids (aa) in length. It contains one kinase domain (aa27­615), and two potential acetylation sites at aa70 and 71. There are four potential splice forms. Two have alternative start sites at aa460 and aa315, while two others show a deletion of aa34­546, plus a three aa substitution for aa 85­204, respectively. Over aa551­622, mouse PCK1 shares 93% and 82% aa identity with rat and human PCK1, respectively. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1ug/ml, Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.