Anti-PBEF1, NT (Pre-B-Cell Colony Enhancing Factor-1, Visfatin)

Visfatin is pre-B cell colony-enhancing factor 1(PBEF), which is predominantly secreted from visceral adipose tissue both in humans and mice. This protein has also been reported to be a cytokine (PBEF) that promotes B cell maturation and inhibits neutrophil apoptosis, or a visceral-fat derived hormone that acts by binding and activating the insulin receptor. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: ELISA (Direct and Indirect): 1:2000-1:5000, Western Blot: 1:2000-1:5000 using ECL. Suggested blocking and dilution buffer is PBST with 0.05% Tween 20 with 5% skim milk. Incubation time: 1 hour at RT., Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.