Anti-p53 (Tumor Suppressor Protein, Oncogene Protein), clone 3G227

p53 is a 339 amino acid protein (53kD) that is thought to act as a tumor suppressor gene. p53 is a DNA-binding protein, and missense mutation of the gene can lead to loss of its DNA-binding functions, and subsequent inactivation of p53 may be a contributing factor in tumorigenesis. Mutation of p53 occurs at many sites and represents a common genetic alteration in human malignancy. Mutations which result in overexpression of p53 can be detected by immunohistochemistry, and overexpression and accumulation of p53 in cell nucleus was reported for a number of human tumors, such as breast, lung and colon carcinomas. Staining of p53 overexpression may be a useful tumor and prognostic marker. Applications: Suitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: ~1:1000, Immunoprecipitation (Native): ~2-5ug, Immunohistochemistry (Frozen or PE): ~1:50-100, Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.