Anti-p53 (Antigen NY-CO-13, Cellular Tumor Antigen p53, Phosphoprotein p53, Tumor Suppressor p53, TP

The p53 gene is located on human chromosome 17p13.1. Mutation and/or allelic loss of this gene is one of the causes of the occurrence of a variety of mesenchymal and epithelial tumors. If it occurs in the germ line, such tumors run in families. P53 binds to a DNA consensus sequence, the p53 response element, and it regulates normal cell growth cycle events by activating transcription of genes, involved either in progression through the cycle, or causing arrest in G1 when the genome is damaged. In most transformed and tumor cells the concentration of p53 is increased 5?1000 fold over the minute concentrations (1000 molecules cell) in normal cells, principally due to the increased half?life (4 h) compared to that of the wild?type (20 min). P53 localizes in the nucleus, but is detectable at the plasma membrane during mitosis and when certain mutations modulate cytoplasmic/nuclear distribution. P53 is the most commonly mutated gene in spontaneously occurring human cancers. Mutations arise with an average frequency of 70% but incidence varies from zero in carcinoid lung tumors to 97% in primary melanomas. High concentrations of p53 protein are transiently expressed in human epidermis and superficial dermal fibroblasts following mild ultraviolet irradiation. Applications: Suitable for use in Flow Cytometry, Immunohistochemistry and Western Blot. Other applications not tested. Recommended Dilution: Immunohistochemistry: Frozen, paraffin., Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.