Anti-Nampt (Visfatin/PBEF, PBEF1, Pre-B Cell Colony Enhancing Factor 1, Nicotinamide Phosphoribosylt

Nicotinamide phosphoribosyltransferase (Nampt, pre-B cell colony-enhancing factor, PBEF, Visfatin) is an 52kD adipokine secreted by adipose tissue and involved in the biosynthesis of nicotinamide adenine dinucleotide (NAD+). Two forms of Nampt exist, an intracellular form (iNampt) and an extracellular form (eNampt). While the function of iNampt as an essential and rate-limiting NAD+ biosynthetic enzyme is well established, the physiological role of eNampt is still a matter of debate. Nampt has various functions, including the promotion of vascular smooth muscle cell maturation and inhibition of neutrophil apoptosis. It activates insulin receptor and has insulin-mimetic effects, lowering blood glucose and improving insulin sensitivity. The protein is highly expressed in visceral fat and serum levels of the protein correlate with obesity. Applications: Suitable for use in ELISA, Flow Cytometry, Immunocytochemistry, Immunohistochemistry, Immunoprecipitation and Western Blot. Other applications not tested. Recommended Dilutions: ELISA (Direct or Indirect): 1:2000-1:10,000, Flow Cytometry: 1:1000, Immunocytochemistry: 1:500-1:1000, Immunohistochemistry (Paraffin): 1:500-1:1000, Immunoprecipitation: 1:500-1:1000, Western Blot: 1:2000-1:5000. Suggested blocking and dilution buffer is PBST containing 0.05% Tween-20, 5% skim milk, suggested incubation time is 1 hour at RT., Optimal dilutions to be determined by researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.