Anti-MSLN (Mesothelin, CAK1 Antigen, MPF, Pre-pro-megakaryocyte-potentiating Factor, SMR)

Megakaryocyte Potentiating Factor (MPF) is a 33kD soluble protein that is cleaved as an N-terminal propeptide from the mouse mesothelin preproprotein by furin, while the C-terminal portion is processed further to form mesothelin. Mouse MPF potentiates megakaryocyte colony forming activity of IL-3 in mouse bone marrow culture. Human MPF shows 55% aa identity with mouse or rat MPF. The preproprotein is produced by mesothelial cells and is also highly expressed in pancreatic, gastric and ovarian carcinomas. Applications: Suitable for use in ELISA, Western Blot and Simple Western. Other applications not tested. Recommended Dilution: Western Blot: 2ug/ml under reducing conditions of human ovarian cancer tissue lysates, Simple Western: 20ug/ml under reducing conditions of human ovarian cancer tissue lysates and human lung tisses, Optimal dilutions to be determined by the researcher. Hybridoma: Fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, NS0-derived, recombinant human MPF. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.