Anti-Macrophage, Monocyte Chemotactic Protein-1 (MCP1, CCL2, JE)

MCP-1 (Monocyte Chemoattractant Protein 1), otherwise known as CCL2, is a 76aa CC chemokine which acts as a chemoattractant for monocytes, basophils, activated T cells and NK cells, through binding with the seven-transmembrane G protein-coupled receptor, CCR2. The recruitment of mononuclear cells by MCP-1, has established this chemokine as an important factor in innate immunity, implicated in the pathogenesis of inflammatory diseases such as rheumatoid arthritis, multiple sclerosis, uveitis and bronchial asthma. Increasing studies have also linked MCP-1 with the polarization of activated T cells into the T helper 2 (Th2) subset, revealing a role for MCP-1 in the adaptive immune response. Applications: Suitable for use in ELISA, Western Blot and Functional Assays. Other applications not tested. Recommended Dilution: ELISA: 0.5ug/ml, Western Blot: 0.1-0.2ug/ml, Functional Assays: 2-3ug/ml, Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.