Anti-LUCIFERASE, Peroxidase Conjugated

Anti-Luciferase Peroxidase Conjugated is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum as well as purified and partially purified Luciferase [Photinus pyralis (Firefly)].  No reactivity is observed against Sea pansy (Renilla reniformis) luciferase. Anti-Luciferase Peroxidase conjugated antibody is produced from Photinus pyralis (Firefly) which produces a green light with a wavelength of 562 nm. Expect a band at approximately 60.7 kDa in size corresponding to Luciferase by western blotting in the appropriate cell lysate or extract.