Anti-Jagged2 (JAG2, HJ2) (APC)

Human Jagged 2 is a 131kD (predicted) type 1 transmembrane protein belonging to the Delta/Serrate/Lag2 (DSL) family of ligands. This family activates LIN12/Notch proteins and thereby regulates cell fate determination during development. There are two isoforms of human Jagged 2, named long and short. The short form lacks a splicing variant region (aa421-461) that is present in the long form of the protein. Human Jagged 2 shares 90%, 87% aa sequence identity with mouse and rat Jagged 2, respectively. During murine embryonic development, Jagged 2 is predominantly expressed in the fetal thymus, epidermis, foregut, dorsal root ganglia, and inner ear. In 2 week old mice, the Jagged 2 transcript is most abundant in heart, lung, thymus, skeletal muscle, brain, and testis. Functionally, it is suggested that Jagged 2 engages the Notch1 pathway of signal transduction. It is involved in the development of the mammalian limb, branchial arches, central and peripheral nervous systems, T cell lineage differentiation, natural killer cells, and the establishment of functional natural killer cell lines. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Hybridoma: NSO myeloma cells with B cells from Balb/c mice. Absorption: 620-650nm , Emission: 660-670nm, Storage and Stability: May be stored at 4°C before opening. DO NOT FREEZE! Stable at 4°C as an undiluted liquid. Dilute only prior to immediate use. Stable for at least 12 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer. APC is light sensitive.