Anti-Histone H3K27Me3 Monoclonal Antibody (Clone RM175)

Histone H3 is a nuclear protein and a component of the nucleosome core, a basic unit of chromatin, that is essential for organizing genomic DNA in eukaryotic nuclei. It is a globular protein that contains an unstructured N-terminal tail that extends outside of the nucleosome core and is subject to various post-translational modifications (PTMs), including methylation, phosphorylation, acetylation, and citrullination. Trimethylation of histone H3 at lysine 27 (H3K27Me3) is associated with gene silencing. It is involved in tumor progression through its regulation by enhancer of zeste homolog 2 (EZH2) and transcriptional repression of tumor suppressor genes. Levels of H3K27Me3 are reduced in 293 T-REx cells containing EEDR236T and SUZ12G610V mutations and in lymphoblastoid cells isolated from patients with Weaver syndrome, a rare overgrowth disorder characterized by EZH2, EED, or SUZ12 mutations, cancer susceptibility, and various distinctive physical features. Cayman's Histone H3K27Me3 Monoclonal Antibody can be used for chromatin immunoprecipitation (ChIP), ELISA, immunohistochemistry (IHC), multiplex-based assay, and Western blot (WB) applications.Synonyms: Trimethylated Histone H3 Lysine 27. Immunogen: Peptide corresponding to H3K27Me3. Clone Designation: RM175. Formulation: (Request formulation change), 100 µg of protein A-affinity purified monoclonal antibody. Storage Buffer: PBS with 50% glycerol, 1% BSA, and 0.09% sodium azide. Host: Rabbit. Isotype: IgG. Applications: ChIP, ELISA, IHC, multiplex-based assays, WB. Cross Reactivity: (+) H3K27Me3, (-) Unmodified histone H3K27, (-) H3K27Me1, (-) H3K27Me2, (-) H3K4Me1, (-) H3K4Me2, (-) H3K4Me3, (-) H3K9Me1, (-) H3K9Me2, (-) H3K9Me3, (-) H3K14Me2, (-) H3K18Me1, (-) H3K18Me2, (-) H3K23Me1, (-) H3K23Me2, (-) H3K36Me1, (-) H3K36Me2, (-) H3K36Me3, (-) H3K56Me1, (-) H3K79Me1, (-) H3K79Me2, (-) H3K79Me3.