Anti-Histone H3.3 Monoclonal Antibody (Clone RM190)

Histone H3 is a nuclear protein and a component of the nucleosome core, a basic unit of chromatin, that is essential for organizing genomic DNA in eukaryotic nuclei. It is a globular protein that contains an unstructured N-terminal tail that extends outside of the nucleosome core and is subject to various post-translational modifications (PTMs). Metazoans have three histone variants: canonical histone H3.1, DNA replication-independent histone H3.3, and the centromeric variant CENP-A. Histone H3.3 is incorporated into chromatin in a DNA replication-independent manner at sites of active transcription. Mutations in H3F3A, the gene encoding histone H3.3 in humans, at or close to lysine 27 or glycine 34 induce upregulation of the oncogene MYCN and are correlated with poor survival in patients with pediatric glioblastoma. Cayman's Histone H3.3 Monoclonal Antibody (Clone RM190) can be used for ELISA, multiplex-based assays, and Western blot (WB) applications.Synonyms: H3.3A, H3F3. Immunogen: Peptide corresponding to human histone H3.3. Clone Designation: RM190. Formulation: (Request formulation change), 100 µg of protein A-affinity purified monoclonal antibody. Storage Buffer: PBS with 50% glycerol, 1% BSA, and 0.09% sodium azide. Host: Rabbit. Isotype: IgG. Applications: ELISA, multiplex-based assays, WB. Cross Reactivity: (+) H3.3 independent of PTMs, (-) H3.1, (-) Other histone proteins.