Anti-Heme Oxygenase 1 (HO-1, HSP32, Heat Shock Protein 32)

Heme Oxygenase (HO) catalyzes the NADPH, O2 and cytochrome P450 reductase dependent oxidation of heme to carbon monoxide, iron and biliverdin. These products of the HO reaction render important physiological effects. Carbon monoxide becomes a potent vasodilator, biliverdin and its product bilirubin function as potent antioxidants, and 'free' iron increases oxidative stress and regulates the expression of many mRNAs by affecting the conformation of iron regulatory protein-1 and its binding to iron regulatory elements in the 5' - or 3'-UTRs of the mRNAs. To date, researchers have identified heme oxygenase isoforms HO-1, HO-2 and HO-3. The mRNA and activity of HO-1/Hsp32, a ubiquitous major heat shock/stress response protein, can be increased several-fold by heme, other metalloporphyrins, transition metals and stimuli that induce cellular stress. The 5'-untranslated region (UTR) of HO-1 contains several consensus regulatory elements. HO-1 expression increases in benign prostatic hyperplasia and malignant prostate tissue, suggesting a role for this stress protein in the pathogenesis of BPH and prostate cancer. Recent data demonstrates the ability of Peroxynitrite (ONOO-) to modulate HO-1 expression, suggesting that the heme oxygenase pathway contributes to protection against the cytotoxic action of ONOO-. ONOO- rapidly decomposes to a highly reactive hydroxyl radical and nitrogen dioxide, both of which cause oxidative damage., , Applications:, Suitable for use in ELISA, Western Blot, Immunohistochemistry and Flow Cytometry. Other applications have not been tested. Recommended Dilutions: Western Blot: 1:1000, Flow Cytomtery: 1:100, Optimal dilutions to be determined by researcher. Positive Control: H1847-67: Heme Oxygenase 1, Recombinant, Rat, Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.