Anti-Heat Shock Protein 22 (HSPB8, HSP22, CRYAC)

Heat shock protein beta-8 (HSPB8, also HSP22 or CRYAC) is a 22-25kD member of the small HSP (HSP20) family of proteins. It is expressed in muscle (smooth, skeletal and cardiac), and serves as a molecular chaperone. Human HSPB8 contains one alpha-crystalline domain (aa 93-170) that mediates protein-protein interaction, and N- plus C-terminal flanking sequences that generate homodimers, homooligomers, and heterodimers with HSP27 and HSPB7. Phosphorylation at Ser57 blocks HSPB8 chaperone activity. Human HSPB8 shares 94% aa identity with both mouse and canine HSPB8. Deficiencies in HSPB8 are involved in distal motor neuropathy type 11A. Applications: Suitable for use in Western Blot. other applications not tested. Recommended Dilution: Western Blot: 0.5ug/ml., Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute by adding sterile buffer. Aliquot and store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.