Anti-GABA Receptor A, alpha1 (Gamma-aminobutyric Acid Receptor Type A alpha 1 Subunit, GABRA1, ECA4,

Gamma-aminobutyric acid (GABA) is the primary inhibitory neurotransmitter in the central nervous system, causing a hyperpolarization of the membrane through the opening of a Cl- channel associated with the GABA-A receptor subtype. GABA-A receptors are important therapeutic targets for a range of sedative, anxiolytic, and hypnotic agents and are implicated in several diseases including epilepsy, anxiety, depression, and substance abuse. The GABA-A receptor is a multimeric subunit complex. To date six alpha, four beta, and four gamma subunits, plus alternative splicing variants of some of these subunits, have been identified. Chloride conductance of these channels can be modulated by agents such as benzodiazepines that bind to the GABA-A receptor. Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1:500-1:5000, Immunohistochemistry (Paraffin): 10ug/ml, Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile dH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.