Anti-Fibroblast Activation Protein alpha(FAPa, FAP, DKFZP686G13158, EC 3.4.21.-, Seprase)

FAP (Fibroblast Activation Protein alpha, also known as seprase) is a 97kD Type II transmembrane serine protease. FAP degrades gelatin, Type I collagen and N-terminal prolines. Enzymatically active FAP is a dimer that migrates at ~170kD. FAP is induced on reactive stromal fibroblasts in epithelial cancers, sarcomas and granulation tissue, and may play roles in tumor invasion, tissue remodeling and wound repair. The 760 amino acid (aa) human FAP contains a 735aa extracellular domain that shares 90% aa identity with mouse or rat FAP. A reported 672aa splicing variant diverges prior to the active site charge relay residues at the C-terminus. Applications: Suitable for use in Flow Cytometry and ELISA. Other applications not tested. Recommended Dilutions: Flow Cytometry: .0.25ug labels 1x10e6 WI-38 human lung fibroblast cell line , ELISA: 2-8ug/ml. Can be used in combination with biotinylated human FAP detection antibody, Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.