Anti-Dopamine Decarboxylase (DOPA)

Dopa decarboxylase (DDC), also known as AADC, is a homodimeric vitamin B 6-dependent enzyme that catalyzes the decarboxylation of hydroxylated aromatic amino acids (Accession # P20711). DDC is expressed in the adrenal medulla and catecholaminergic neurons. It catalyzes the final step in the synthesis of the neurotransmitters dopamine and serotonin. Defects in DDC are associated with the development of schizophrenia, Parkinson's disease, and hypertension. Human DDC shares 89% aa sequence identity with mouse and rat DDC. Applications: Suitable for use in Western Blot and ELISA. Other applications not tested. Recommended Dilution: Western Blot: 2ug/ml. Detects human DDC in cell or tissue extracts, Direct ELISA: 0.5-1ug/ml, The detection limit is ~2ng/well., Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.