Anti-Detyrosinated alpha-Tubulin Rabbit Monoclonal Antibody (Clone RM444)

Detyrosinated alpha-tubulin is a form of the cytoskeletal protein alpha-tubulin that has been post-translationally modified by the tubulin carboxypeptidases vasohibin 1 (VASH-1) and VASH-2, which remove the C-terminal tyrosine from alpha-tubulin. Detyrosination of alpha-tubulin occurs in the cytosol and exposes two consecutive glutamate residues that can be further processed to form Delta2- or Delta3-tubulin. Detyrosinated alpha-tubulin monomers can be re-tyrosinated by tubulin tyrosine ligase and be incorporated into microtubules again. alpha-Tubulin detyrosination is associated with, but not sufficient for, increased stability of the microtubule and is involved in a variety of biological processes, including neuronal development, beta-oxidation, mitosis, and cardiomyocyte contraction. Increased levels of detyrosination are associated with tumor development in vitro, have been found in tumor tissue from patients with breast cancer, and are positively correlated with breast cancer aggressiveness. Cayman's Detyrosinated alpha-Tubulin Rabbit Monoclonal Antibody (Clone RM444) can be used for immunocytochemistry (ICC), immunofluorescence (IF), and Western blot (WB) applications.Synonyms: Glu-tubulin. Immunogen: A peptide corresponding to detyrosinated alpha-tubulin. Clone Designation: RM444. Formulation: (Request formulation change), 100 µl of protein A-affinity purified monoclonal antibody.. Storage Buffer: PBS with 50% glycerol, 1% BSA, and 0.09% sodium azide. Host: Rabbit. Isotype: IgG. Applications: ICC, IF, WB. Cross Reactivity: (+) Detyrosinated alpha-tubulin.