Anti-CYP1B1 (Cytochrome P450 1B1, CYPIB1, Cytochrome P450CMEF, Cytochrome P450EF, Cyp1-b1)

Cytochrome P450 (P450 or CYP) enzymes, a superfamily of btype heme-containing proteins found in organisms from all domains of life, are major catalysts in the oxidative transformation of a diversity of endogenous and exogenous compounds. CYP enzymes play an important role in the metabolic activation of environmental procarcinogens or chemical carcinogenesis, these enzymes are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. The enzyme localizes to the endoplasmic reticulum and metabolizes procarcinogens such as polycyclic aromatic hydrocarbons and 17beta-estradiol. Mutations in this gene have been associated with primary congenital glaucoma, therefore it is thought that the enzyme also metabolizes a signaling molecule involved in eye development, possibly a steroid. CYP1B1 (Cytochrome P450 family 1, subfamily B, polypeptide 1) a 543aa enzyme in mouse, rat and human( chr:2p22) belongs to a multigene superfamily of monomeric mixed-function monooxygenases, responsible for the phase 1 metabolism of a wide range of structurally diverse substrates by inserting 1 atom of atmospheric oxygen into the substrate molecule, thereby creating a new functional group (e.g., -OH, -NH2, -COOH). This enzyme is involved in an NADH-Dependent electron transport pathway, It oxidizes a variety of structurally unrelated compounds and participates in the metabolism of an as-yet unknown biologically active molecule that is a participant in eye development. Cyp1B1 is expressed in many tissues, Defects in Cyp1B1 causes primary congenital Glaucoma, this recessive disease is characterized by large ocular globes resulting from increased intraocular pressure. , Applications:, Suitable for use in ELISA. Western Blot, though not tested, may potentially be used as an application. Other applications not tested. Recommended Dilution: ELISA: 1:100,000, Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.