Anti-COX-2 (Clone 12C10)

Cyclooxygenase 2 (COX-2) is a bifunctional enzyme that exhibits both COX and peroxidase activities and catalyzes the first step in the biosynthesis of prostaglandins, thromboxanes, and prostacyclins. The COX component converts arachidonic acid (Cay-90010, Cay-90010.1, Cay-10006607) to the hydroperoxy endoperoxide prostaglandin G2 (PGG2, Cay-17010), and the peroxidase component reduces the endoperoxide to the corresponding alcohol PGH2 (Cay-17020). COX2 expression is induced by a variety of stimuli, including phorbol esters, LPS, and cytokines and is responsible for the biosynthesis of PGs under acute inflammatory conditions. Thus, COX-2 has been the focus of attention for nonsteroidal anti-inflammatory drug (NSAID) development. Cayman's COX-2 Monoclonal Antibody (Clone 12C10) can be used for ELISA, flow cytometry (FC), immunofluorescence (IF), and Western blot (WB) applications. The antibody recognizes a unique C-terminal region of COX-2 that is not present in COX-1, specifically detecting COX-2 at 72 kDa from human, mouse, and ovine samples.Synonyms: Cyclooxygenase-2, PGHS-2, Prostaglandin H Synthase 2. Immunogen: Synthetic peptide from the C-terminal region of mouse COX-2. Clone Designation: 12C10. MW: 72 kDa. Formulation: (Request formulation change), 50 µg of protein G-purified IgG. Storage Buffer: PBS, pH 7.2, with 50% glycerol, 0.1% BSA, and 0.02% sodium azide. Host: Mouse. Isotype: IgG1. Applications: ELISA, FC, IF, WB. Cross Reactivity: (-) COX-1 (all species). UniProt Accession #: P35354.