Anti-cdk1, amino acids 263-287 (cdc2, PSTAIR, Cyclin-dependent Kinase 1)

Entry of all eukaryotic cells into mitosis is regulated by activation of CDC2 kinase. Activation of CDC2 is controlled at several steps including cyclin binding and phosphorylation of Thr161. However, the critical regulatory step in activating CDC2 during progression into mitosis appears to be dephosphorylation of Tyr15 and Thr14. Phosphorylation at Tyr15 and inhibition of CDC2 is carried out by Wee1 and Myt1 protein kinases, while Tyr15 dephosphorylation and activation of CDC2 is carried out by the CDC25 phosphatase. Expression of CDC2 plays a critical role in cell transition through the G2/M phase. The activity of CDC2 in dividing cells increases at the onset of M phase coincident with disassembly of the cell nucleus, generation of mitotic spindles and chromosome condensation. Several proteins including Histone H1 and pp60src are substrates for CDC2. Applications: Suitable for use in ELISA, Western Blot, and Immunoprecipitation. Other applications have not been tested. Recommended Dilution: Western Blot: 1:250-1:1000 detects cdk1/cdc2 in RIPA lysates from 3T3 cells. 3T3 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with C2571-06 (1:2000). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system., Immunoprecipitation: 10ul immunoprecipitates cdk1/cdc2 from 500ug of 3T3 RIPA lysate. , Optimal dilutions to be determined by the researcher. Positive Antigen Control: 3T3 cell lysate, use 20ug per lane for minigels. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.