Anti-CD80 (B7-1 Costimulator Protein) (azide free), clone 3H1689

CD80, also known as BB1/B7-1, is a 60kD type I transmembrane glycoprotein and a member of the immunoglobulin superfamily of cell surface receptors. It is expressed on activated B cells, T cells, monocytes and dendritic cells. It is a ligand of the two T cell costimulatory molecules, CD28 and CD152 (CTLA-4). The interaction of CD80 (or CD86) with CD28 provides a costimulatory signal for T cells activated through the CD3/TCR complex. Applications: Suitable for use in Flow Cytometry, Western Blot, Immunoprecipitation. Other applications not tested. Recommended Dilution: Western Blot: non-reducing conditions, Flow Cytometry: Use 10ul to label 10e6 cells or 100ul whole blood., Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.