Anti-CD70 (Tnfsf7, CD27 Ligand)

CD27 Ligand is a member of the tumor necrosis factor superfamily (TNFSF7) and is also designated CD70. CD27 Ligand is a type II transmembrane protein expressed by activated T and B cells. It binds to CD27 on mature T cells and a subset of thymocytes. CD27 Ligand induces the proliferation of costimulated T cells and enhances the generation of cytolytic T cells. Applications: Suitable for use in Direct ELISA, Immunocytochemistry and Western Blot. Other applications not tested. Recommended Dilutions: Immunocytochemistry: 8-25ug/ml labels immersion fixed human peripheral blood mononuclear cells, Western Blot: 1ug/ml using recombinant human CD27 Ligand/TNFSF7., Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.