Anti-CD31 (PECAM-1, Platelet gpIIa Molecule), clone 5G2

PECAM-1 is typically an antigen that is shared by both endothelial and distinct hematopoietic cells. It is widely expressed among leukocytes and functions as cell adhesion molecule for these cells. PECAM-1 reactivity can be interpreted as a further evidence for megakaryocytic nature of distinct cell lines has been shown. However, a new useful application for this monoclonal antibody appears to be its very high reactivity against human endothelial cells, , Applications:, Suitable for use in Immunohistochemistry, Western Blot and other immunoassays for cellular and tumor biology. Other applications not tested. Recommended Dilution: Western Blot: 1:500-1:2500, Optimal dilutions to be determined by the researcher. Hybridoma: Derived from the hybridoma produced by fusion between myeloma cells and Balb/c spleen cells. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.,