Anti-CD31 (PECAM-1, Platelet gpIIa Molecule), clone 0.N.100

CD31 (PECAM-1, Platelet gpIIa Molecule), a ~130kD integral membrane glycoprotein, is expressed in platelets, cultured endothelial cells, monocytes, normal tissue macrophages in tonsil, lung and kidney, skin biopsies from conditions such as sarcoidosis and lepromatous leprosy and bone marrow-derived hematopoietic stem cells and embryonic stem cells. CD31 mediates cell-cell adhesion. Valuable iin the study of benign and malignanat vaascular tumors. Stainiing for CD31 has also been used to measure angiogenesis, which reportedly predicts tumor recurrence. Applications: Suitable for use in ELISA, Immunofluorescence and Immunohistochemistry. Not suitable for Western Blot. Other applications have not been tested. Recommended Dilution: Immunohistochemistry (Formalin/paraffin): 1:50 for 30min at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 1mM EDTA, pH 8.0 for 10-20 min., followed by cooling at RT for 20 min., Optimal dilutions to be determined by the researcher. Positive Control: Placenta, Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.