Anti-CD3, epsilon (Early T Cell Marker), FITC conjugated, clone 5K4

CD3epsilon, a member of the immunoglobulin superfamily of cell surface receptors, is comprised of five invariable chain ranging in size from 16-28kD and is closely associated with the T cell antigen receptor (TCR). It is expressed on all T cells of all mouse strains. CD3 plays a major role in signaling during antigen recognition, leading to T-cell activation. In the presence of Fc receptor-bearing accessory cells, soluble antibody can activate primed and naïve T cell in vitro. Immobilized monoclonal antibody can also activate both normal T lymphocytes and cloned T cell lines provided the appropriate accessory signals are present. Applications: Suitable for use in Flow Cytometry, Immunoprecipitation and Immunohistochemistry. Other applications have not been tested. , Recommended Dilutions: Flow Cytometry: <3ug/10e6 cells, Immunohistochemistry: Paraffin, frozen sections, Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. , Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.