Anti-CD3, epsilon (Early T Cell Marker), clone 3N1

Monoclonal antibodies clustered as CD3 recognize the constant structure of the CD3/TcR (T cell receptor) complex. This complex is present on mature T cells and during thymopoiesis. CD3/TcR is called the T cell receptor complex. This complex is composed of different structures, i.e. TcRa, TcRb, TcRg, TcRd, CD3g, CD3d, CD3e, the x-chain, and the h-chain. Less than 10% of human peripheral T cells express the g/d TcR complex. The function of a/b and g/d heterodimers is the recognition of peptide antigen bound to MHC, followed by T cell activation. Recent data suggest that CD3 chains are able to function autonomously in signal transduction. T cells play a major role in activation processes of the immune system. Detection of these cells is important during immune monitoring. In addition, a large number of leukemias are T cell-related disorders, in which antibodies recognizing CD3/TcR complex are important diagnostic tools. Differentiation between AML, T-ALL and B-ALL can be made by immunophenotyping with (early) differentiation markers. Cell surface detection using CD3 antibodies can be applied for detection of mature T-ALL, while immature T-ALL express cytoplasmic (cyCD3) but no surface CD3. CD3 antibodies are used, in characterization of various subtypes of chronic lymphoid leukemias. Examples of these chronic T cell leukemias are T-CLL (Sézary Syndrome) and the peripheral T cell lymphoma (ATLL) which co-express CD2, CD3, CD4 and CD5 antigens. Applications: Suitable for use in Immunohistology (formalin/paraffin). Other applications have not been tested. Recommended Dilution: Immunohistology: 1:150 for 10 min at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 min. followed by cooling at RT for 20 min., Optimal dilutions to be determined by the researcher. Positive Control: Tonsil, Cellular Localization: Cell membrane, Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.