Anti-CD3 (Early T Cell Marker), clone 3H79

The CD3 complex is closely associated at the lymphocyte cell surface with the T cell antigen receptor (TCR). Reportedly, CD3 is involved in signal transduction to the T cell interior following antigen recognition. The CD3 antigen is first detectable in early thymocytes and probably represents one of the earliest signs of commitment to the T cell lineage. In cortical thymocytes, CD3 is predominantly intracytoplasmic. However, in medullary thymocytes, it appears on the T cell surface. CD3 is a highly specific marker for T cells and is present in majority of T cell neoplasms. CD3 antigen is expressed on ~60-80% of human peripheral blood lymphocytes, 20-40% of splenic lymphocytes, the majority of T-CLL and ~70% pf T-All. Applications: Suitable for use in ELISA and Immunofluorescence. Other applications not tested. Recommended Dilutions: Immunofluorescence: 1:400 stains human peripheral blood lymphocytes., Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.