Anti-CD273 (CD273 Antigen, B7-DC, bA574F11.2, Btdc, Butyrophilin B7-DC, Butyrophilin B7DC, MGC142238

CD273 is a 42kD member of the B7 family, otherwise known as B7-dendritic cell (B7-DC), or programmed death ligand-2 (PD-L2). The expression of CD273 is restricted to a subpopulation of dendritic cells (DCs), and DCs and macrophages that have been stimulated with interferon gamma, GM-CSF or IL-4. CD273 has been identified as a ligand for PD-1(CD279), along with another B7 family member B7-H1 (CD279). Studies suggest that both CD273 and B7-H1 co-stimulate or inhibit T cell proliferation and cytokine production. Applications: Suitable for use in Flow Cytometry, Immunohistochemistry and Immunoprecipitation. Other applications not tested. Recommended Dilutions: Flow Cytometry: 1:100-1:200, 10ul labels 10e6 cells in 100ul, Optimal dilutions to be determined by the researcher. Hybridoma: P3U1 myeloma cells with lymph node cells from SD rats. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.