Anti-CD2 (CD2 Antigen, LFA3 Receptor, Lymphocyte Function Antigen 2, LFA2, p50, Rosette Receptor, Sh

CD2 a well known T-cell marker is a surface antigen of the human T-lymphocyte lineage. Also known as the sheep erythrocyte receptor, CD2 is a 351aa containing protein that contains an Ig-like C2 -type (immunoglobulin like) domain, an Ig-like V -type (immunoglobulin like) domain and a cytoplasmic domain implicated in T cell signaling. It interacts with CD2AP (CD2-associated protein) and PSTPIP1 (proline-serine-threonine phosphatase interacting protein 1). CD2 mediates adhesion between T-cells and other cell types by interacting with lymphocyte function-associated antigen (LFA-3) and CD48/BCM1. Interaction between CD2 and its counter receptor, LFA3 (CD58), on opposing cells helps in immune recognition and facilitates contacts between helper T lymphocytes and antigen-presenting cells as well as between cytolytic effectors and target cells. CD2 expression promotes chronic HBV infection and may also contribute to the hepatocyte damage. Expression of CD2 is common on all peripheral blood T cells, thymocytes, and also on some natural killer cells but not on B lymphocytes. Applications: Suitable for use in Western Blot, Flow Cytometry, Neutralization and Immunohistochemistry. Other applications not tested. Recommended Dilution: Flow Cytometry: 0.1-0.25ug/10e6 cells, Immunohistochemistry (frozen): 1:10-1:500, Western Blot: 1:100-1:2000, Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.