Anti-CD1c (Cluster of differentiation antigen 1c, CD1c antigen, CD1c antigen c polypeptide, CD1c mol

CD1c (Cluster of differentiation antigen 1c) is a 43kD member of the CD1 family of molecules. It is expressed by thymocytes, dendritic cells and B cells, and exists as part of a noncovalent complex with 12kD B2-microglobulin. It is found in the plasma membrane and early endosomes (but not lysosomes), and is presumed to present glycolipids and acylated peptides to T cells. Mature human CD1c is a 316aa type I transmembrane glycoprotein. It contains a 285aa extracellular domain (ECD) (aa18-302) plus a 10aa cytoplasmic tail. The ECD shows one Ig-like domain (aa203-296) that associates with B2-microglobulin, and a TyrGlnAspIle internalization motif in the cytoplasmic tail. There are three potential splice variants. One shows a Trp substitution for aa327-333, a second shows an eight aa substitution for aa298-333, and a third shows a 50aa substitution for aa297-333. There appears to be no direct mouse counterpart to human CD1c. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: Direct ELISA: 0.5-1ug/ml. The detection limit for recombinant human CD1c is ~0.5ng/ml., Western Blot: 1ug/ml, Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.