Anti-CD152, Blocking (CTLA4, Cytolytic T-lymphocyte-associated Antigen 4)

Human CD152 (CTLA-4) is a cell surface glycoprotein expressed at low levels on activated T-cells. CD152 is a high affinity receptor for the costimulatory molecules CD80 (B7-1) and CD86 (B7-2) and appears to function as a negative regulator of T cell activation. Applications: Suitable for use in Flow Cytometry, ELISA and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Flow Cytometry (Blocking): 5ug/ml, Optimal dilutions to be determined by the researcher. Flow Cytometry (Blocking) Protocol: C2548-83C Anti-CD152 antibody blocked the binding of C2548-83V3 CD152 Human, IgG2a Mouse Fc, Fusion Protein (PE) to CD80 and CD86 expressed on human Raji cells. , 1. Five x 10e5 cultured Raji human tumor cells were washed and incubated 45 minutes on ice with 80ul of C2548-83V3 at a concentration of 1ug/ml. , 2. Cells were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 1.79 log10 fluorescent units when compared to a Mouse IgG2a Isotype control (PE) at a similar concentration. Binding was 98.6% blocked when C2548-83V3 CD152 Human, IgG2a Mouse Fc, Fusion Protein (PE) was pre incubated 10 minutes with a five fold excess (w/w) of C2548-83C CD152, Blocking (5ug/ml). Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.