Anti-CCR1 (Chemokine Receptor 1, CC-CCR-1) (APC)

CCR1 (C-C chemokine receptor 1, also known as MIP-1a receptor and CD191) is a 43kD member of the G protein-coupled receptor 1 family of transmembrane proteins. Mouse CCR1 is expressed on osteoclasts, IL-13+ T cells, neutrophils, bone marrow-derived mast cells, eosinophils, monocytes, and vascular intimal smooth muscle cells. Multiple chemokines are reported to bind to CCR1. MIP-1a/CCL3 and RANTES/CCL5 are natural ligands, while CCL15, CCL9, and CCL23 are potent agonists after N-terminal processing. Mouse CCR1 is a seven transmembrane protein that is 355aa in length. It contains a 34aa N-terminal extracellular domain plus a 50aa C-terminal cytoplasmic tail. Over aa sequences 1-34, 92-107, 172-192, and 265-281 collectively, mouse CCR1 shares 76% and 89% aa identity with human and rat CCR1,respectively. Applications: Suitable for use in Direct ELISA, Western Blot and Flow Cytometry. Other applications have not been tested. Recommended Dilutions: Flow Cytometry: 10ul/10e6 using RAW 264.7 mouse monocyte/macrophage cell line and L1.2 mouse pro-B cell line transfected with mouse CCR1 and eGFP, Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.