Anti-Cathepsin K

Human cathepsin K (EC 3.4.22.38) is a member of the cysteine protease (papain) family with high primary sequence homology to cathepsins S, L, and B. It has been shown that cathepsin K plays a major role in the resorption of the bone matrix by osteoclasts. Cathepsin K has a potential as a drug target for the diseases related to bone matrix metabolism such as osteoporosis. Autoproteolytic processing of the N-terminal 99 amino acid propeptide produces the active, mature form of cathepsin K. It is presumed that the activation of procathepsin K in vivo occurs in the bone resorption pit, which has a low-pH environment. Humans lacking cathepsin K exhibit pycnodysostosis, which is characterized by short stature and osteosclerosis. Applications: Suitable for use in Indirect ELISA, Immunohistochemistry and Western Blot. Other applications not tested. Recommended Dilutions: Optimal dilutions to be determined by the researcher. Amino Acid Sequence: MRGSHHHHHH GSLYPEEILD THWELWKKTH RKQYNNKVDE ISRRLIWEKN LKYISIHNLE ASLGVHTYEL AMNHLGDMTS EEVVQKMTGL KVPLSHSRSN DTLYIPEWEG RAPDSVDYRK KGYVTPVKNQ GQCGSCWAFS SVGALEGQLK KKTGKLLNLS PQNLVDCVSE NDGCGGGYMT NAFQYVQKNR GIDSEDAYPY VGQEESCMYN PTGKAAKCRG YREIPEGNEK ALKRAVARVG PVSVAIDASL TSFQFYSKGV YYDESCNSDN LNHAVLAVGY GIQKGNKHWI IKNSWGENWG NKGYILMARN KNNACGIANL ASFPKM, Storage and Stabillty: Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.